small rna expression microarray (8×15k format)  (Arraystar inc)

Journal: bioRxiv

Article Title: Genetic inactivation of the Translin/Trax RNase activity alters small RNAs including miRNAs, disrupts gene expression and impairs distinct forms of hippocampal synaptic plasticity and memory

doi: 10.1101/2025.07.10.663777

Figure Lengend Snippet: Volcano plots showing the mature miRNAs differentially expressed in the TraxE126A mutants compared to wildtype littermates as identified by (A) miRNA sequencing and (B) miRNA microarray. Volcano plots showing the differentially expressed small RNA species identified using microarray analysis including (C) Precursor miRNAs (pre-miRNAs), (D) Small nucleolar RNAs (snoRNAs), (E) mature tRNAs and (F) tRNA-derived small RNAs (tsRNAs). In all the plots, the upregulated and downregulated miRNAs (false discovery rate, FDR <0.050 and log 2 fold change ≥0.200) are highlighted in red and blue, respectively. (TraxE126A, n=4; WT, n=5, all males). Largest changes were seen in tsRNA levels (majority are 5’-fragments) and mature miRNAs.

Article Snippet: The labeled RNA species are then hybridized onto Arraystar Small RNA Expression Microarray (8×15K format), scanned by an Agilent G2505C scanner followed by data processing and analysis.

Techniques: Sequencing, Microarray, Derivative Assay

Journal: bioRxiv

Article Title: Genetic inactivation of the Translin/Trax RNase activity alters small RNAs including miRNAs, disrupts gene expression and impairs distinct forms of hippocampal synaptic plasticity and memory

doi: 10.1101/2025.07.10.663777

Figure Lengend Snippet: (A) Venn diagram showing the overlap between mature miRNAs identified using miRNA sequencing and microarray analysis (with FDR<0.050 and log 2 fold change ≥0.200). A total of 12 miRNAs (10 upregulated and 2 downregulated) were found to be common and were used for target prediction using miRDB database. (B) An upset plot showing the shared and unique predicted mRNA target profiles in the miRDB database for the 12 common miRNAs. Only targets with miRDB Target Score ≥60 are included. (C) Top 15 KEGG pathways and (D) Gene Ontology (GO) Biological Process terms from the functional enrichment analysis of the predicted targets of the 12 common miRNAs performed using DAVID database.

Article Snippet: The labeled RNA species are then hybridized onto Arraystar Small RNA Expression Microarray (8×15K format), scanned by an Agilent G2505C scanner followed by data processing and analysis.

Techniques: Sequencing, Microarray, Functional Assay

Journal: Frontiers in Pharmacology

Article Title: SNORA13 antisense oligonucleotides enhances the therapeutical effects of 5-fluorouracil in colon adenocarcinoma

doi: 10.3389/fphar.2025.1564682

Figure Lengend Snippet: SNORA13 is upregulated in CRC. (A) The volcano plot showed the differentially expressed snoRNAs analyzed by the small RNA array in CRC samples. Red and blue dots represent the upregulated and downregulated snoRNAs, respectively (p < 0.05). SNORA13 is highlighted in red text. (B) A Venn diagram of upregulated snoRNAs in COAD and READ datasets from TCGA and CRC tissues from clinical samples, in which only SNORA13 overlapped. (C) The SNORA13 expression in paired COAD tumor and adjacent non-tumor tissues from TCGA database. (D) The SNORA13 expression in paired READ tumor and adjacent non-tumor tissues from TCGA database. Data are shown as mean ± S.D., (C,D) used paired t -test to perform the statistical significance, three biological replicates for each sample were performed, ** p < 0.01.

Article Snippet: The labeled RNA was hybridized onto Arraystar Human small RNA Microarray (8 × 15K, Arraystar) and the array was scanned by an Agilent Scanner G2505C.

Techniques: Expressing